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PubMed · 6320105

Plasmid mapping computer program.

G P Nolan·C V Maina·A A Szalay
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Abstract

Three new computer algorithms are described which rapidly order the restriction fragments of a plasmid DNA which has been cleaved with two restriction endonucleases in single and double digestions. Two of the algorithms are contained within a single computer program (called MPCIRC). The Rule-Oriented algorithm, constructs all logical circular map solutions within sixty seconds (14 double-digestion fragments) when used in conjunction with the Permutation method. The program is written in Apple Pascal and runs on an Apple II Plus Microcomputer with 64K of memory. A third algorithm is described which rapidly maps double digests and uses the above two algorithms as adducts. Modifications of the algorithms for linear mapping are also presented.

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Base SequenceComputersDNADNA Restriction EnzymesMethodsMicrocomputersPlasmidsSoftwareJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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G P Nolan, C V Maina, A A Szalay. 1984-01-11. Plasmid mapping computer program.. https://doi.org/10.1093/nar%2F12.1part2.717

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