Protonmotive force in giant mitochondria.

M L Campo; H Tedeschi

doi:10.1111/j.1432-1033.1984.tb08147.x

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Protonmotive force in giant mitochondria.

M L Campo·H Tedeschi

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Abstract

The accompanying communication [Eur. J. Biochem. 141 (1984) 1-4] indicates that the microinjection of the pH fluorescent indicator pyranine (8-hydroxy-1,2,6- pyrenesulfonate ) into giant mitochondria or mitoplasts does not affect their ability to carry out oxidative phosphorylation. The dye can therefore be used as a quantitative indicator of internal mitochondrial pH. We found that activation of metabolism in rotenone-inhibited giant mitochondria by the addition of succinate produces an internal pH change corresponding to a pH shift of 0.3 to the alkaline range, approximately the same value found previously for conventional rat liver mitochondria.

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Animals Arylsulfonates Fluorescent Dyes Hydrogen-Ion Concentration In Vitro Techniques Mice Mitochondria, Liver Oxidative Phosphorylation Protons Spectrometry, Fluorescence Journal Article Research Support, U.S. Gov't, P.H.S.

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M L Campo, H Tedeschi. 1984-05-15. Protonmotive force in giant mitochondria.. https://doi.org/10.1111/j.1432-1033.1984.tb08147.x

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