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An Escherichia coli gene required for bacteriophage P2-lambda interference.

D Ghisotti·S Zangrossi·G Sironi
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Abstract

The gene old of bacteriophage P2 is known to (i) cause interference with phage lambda growth; (ii) kill recB- mutants of Escherichia coli after P2 infection; and (iii) determine increased sensitivity of P2 lysogenic cells to X-ray irradiation. In all of these phenomena, inhibition of protein synthesis occurs. We have isolated bacterial mutants, named pin (P2 interference), able to suppress all of the above-mentioned phenomena caused by the old+ gene product and the concurrent protein synthesis inhibition. Pin mutations are recessive, map at 12 min on the E. coli map, and identify a new gene. Satellite bacteriophage P4 does not plate on pin-3 mutant strains and causes cell lethality and protein synthesis inhibition in such mutants. P4 mutants able to grow on pin-3 strains have been isolated.

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ColiphagesEscherichia coliGenes, RecessiveGenotypeLysogenyMutationJournal ArticleResearch Support, Non-U.S. Gov't

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D Ghisotti, S Zangrossi, G Sironi. 1983. An Escherichia coli gene required for bacteriophage P2-lambda interference.. https://doi.org/10.1128/jvi.48.3.616-626.1983

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