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A quantitative microassay for leukocyte chemotaxis, using a microscopic slide system with complement-activating yeast particles as gradient source.

B I Coble·C Dahlgren·J Hed·O Stendahl
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Abstract

A simple quantitative microassay was developed for studying polymorphonuclear leukocyte (PMNL) chemotaxis under conditions where the number of available cells is a limiting factor, e.g., pustules, neutropenia, small children and cerebrospinal fluid. PMNL suspensions are placed on glass slides to which fluorescein-labeled yeast particles have been fixed. After adherence, normal human serum is added to the slides. Owing to complement activation, a chemotactic gradient which attracts the adherent PMNL is formed around the yeast particles. The number of PMNL-associated yeast particles in the presence of normal serum is scored, and compared with cells migrating in the presence of inactivated serum or in the absence of serum. A locomotory index is calculated as the number of yeast particles associated with PMNL divided by the total number of yeast particles.

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Blood Physiological PhenomenaChemotaxis, LeukocyteChildComplement ActivationGranulocytesHumansImmunologic TechniquesMeningitis, MeningococcalNeutrophilsSaccharomyces cerevisiaeSepharoseJournal ArticleResearch Support, Non-U.S. Gov't

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B I Coble, C Dahlgren, J Hed, O Stendahl. 1983-11-25. A quantitative microassay for leukocyte chemotaxis, using a microscopic slide system with complement-activating yeast particles as gradient source.. https://doi.org/10.1016/0022-1759(83)90437-4

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