PubMed · 6381287
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1984. 1,8-Dinitropyrene.. https://pubmed.ncbi.nlm.nih.gov/6381287/
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The mechanism of arsine (AsH3) induced hemolysis was studied in vitro using isolated red blood cells (RBCs) from the rat or dog. AsH3-induced hemolysis of dog red blood cells was completely blocked by carbon monoxide (CO) preincubation and was reduced by pure oxygen (O2) compared to incubations in air. Since CO and O2 bind to heme and also reduce hemolysis, these results suggested a reaction between AsH3 and hemoglobin in the heme-ligand binding pocket or with the heme iron. Further, sodium nitrite induction of methemoglobin (metHb) to 85% and 34% of total Hb in otherwise intact RBCs resulted in 56% and 16% decreases in hemolysis, respectively, after incubation for 4 h. This provided additional evidence for the involvement of hemoglobin in the AsH3-induced hemolysis mechanism. Reactions between AsH3 and hemoglobin were studied in solutions of purified dog hemoglobin. Spectrophotometric studies of the reaction of AsH3 with various purified hemoglobin species revealed that AsH3 reacted with HbO2 to produce metHb and, eventually, degraded Hb characterized by gross precipitation of the protein. AsH3 did not alter the spectrum of deoxyHb and did not cause degradation of metHb in oxygen, but bound to and reduced metHb in the absence of oxygen. These data indicate that a reaction of AsH3 with oxygenated hemoglobin HbO2, may lead to hemolysis, but there are reactions between AsH3 and metHb that may not be directly involved in the hemolytic process.
The genotoxic effects of an average concentration of 41.7 mg/m3 of SO2 exposure on 42 workers of a fertilizer factory were investigated. Mitotic index (MI), chromosomal aberrations (CAs), sister-chromatid exchanges (SCEs) and satellite associations (SA) were observed. In SO2-exposed workers, a higher mitotic index (7.09) was recorded in comparison to controls (4.34). The MI, however, declined with duration of exposure. Satellite associations showed a two-fold increase (17.1) as compared to controls (8.11). Among chromosomes, D-G group associations were the highest (7.43%), while 3D type associations were the lowest (0.4%). There was a significant difference (p < 0.05) in the mean frequency of CAs per cell in the exposed workers (3.262%) and the controls (0.833%). The mean frequency of SCEs per cell increased from 3.32 +/- 0.1 in controls to 7.72 +/- 0.19 in the exposed group. The difference was significant (p < 0.05). In smokers, alcoholics and smoker-alcoholics, the frequency of CAs and SCEs per cell was significantly higher than the non-smokers and non-alcoholics, both in the controls and the SO2-exposed workers and showed a correlation with the duration of exposure. SO2 is therefore a clastogenic and genotoxic agent for which necessary precautions must be taken.
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