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PubMed · 6419401

A simple method for collecting 14CO2 in fish.

B E Bengtsson·I Brandt·P O Darnerud·J Larsson
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Abstract

A simple apparatus was designed for continuous collection of excreted 14CO2 in fish. Carp (Cyprinus carpio; body wt. about 400 g) were intraarterially injected with [14C]acetate (44 mmol) and transferred to a test chamber (21 +/- 1 degrees C). Within 48 h, 65.4 +/- 2.7% of the dose was recovered as 14CO2. A small amount of 14CO2 was retained in the water of the test chamber. Control experiments using [14C]acetate-injected mice as '14CO2-generators' showed that this 14C residue in the water could be recovered almost completely as 14CO2 upon acidification. In the carp experiments, some radioactivity remained in the acidified test chamber water; this residue may represent unchanged [14C]acetate and/or metabolites other than 14CO2.

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AcetatesAcetic AcidAnimalsCarbon DioxideCarbon RadioisotopesCarpsInjections, Intra-ArterialMethodsMiceWaterJournal ArticleResearch Support, Non-U.S. Gov't

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B E Bengtsson, I Brandt, P O Darnerud, J Larsson. 1983. A simple method for collecting 14CO2 in fish.. https://doi.org/10.1016/0378-4274(83)90140-6

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