Spontaneous and conA-induced suppressor lymphocytes: a comparative study.

C Gattringer; H Huber; G Michlmayr; H Braunsteiner

doi:10.1016/s0171-2985(81)80087-3

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Spontaneous and conA-induced suppressor lymphocytes: a comparative study.

C Gattringer·H Huber·G Michlmayr·H Braunsteiner

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Abstract

Suppressor monocytes, Concanavalin A(ConA)-induced suppressor T cells, and short-lived suppressor lymphocytes have been describe in humans. The present study was performed to evaluate spontaneous suppression in a test system similar to that employed for the demonstration of ConA-induced suppressor cells: Lymphocytes were either stimulated by ConA (= induced suppressor cells) or immediately mitomycin-treated (= spontaneous suppressor cells). Both preparations were tested for their capacity to suppress mitogen-induced proliferation of autologous cells. Depletion of monocytes or B lymphocytes did not affect spontaneous suppression. The active cells were short-lived in vitro. Therefore the net increase in suppressor activity generated by preculture with ConA is in part related to a loss of spontaneous inhibitory activity in the control cultures kept without mitogen. Spontaneous suppressor cell activity was comparable to that of ConA-induced suppressor cells.

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Carrageenan Concanavalin A Humans Lymphocyte Activation Lymphocytes Mitomycins Monocytes Phytohemagglutinins Pokeweed Mitogens T-Lymphocytes, Regulatory Time Factors Comparative Study Journal Article Research Support, Non-U.S. Gov't

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C Gattringer, H Huber, G Michlmayr, H Braunsteiner. 1981. Spontaneous and conA-induced suppressor lymphocytes: a comparative study.. https://doi.org/10.1016/s0171-2985(81)80087-3

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