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PubMed · 6469388

An immunochemical method for assessing the function of the alternative complement pathway.

K Yoshida·Y Yukiyama·S Hirose
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Abstract

Activation of the alternative pathway of human complement (C) by soluble C activators resulted in a decrease of C9 antigen, measured by single radial immunodiffusion, concomitant with a decrease of C9 hemolytic activity. In the presence of ethylene glycol bis-(beta-aminoethylether)-tetraacetic acid (EGTA) and Mg2+, this decrease of C9 antigen in the presence of soluble activators such as dinitrophenylated bovine serum albumin depends mainly on the activation of the alternative C pathway. Therefore, an assay system to express the total activity of the alternative C pathway in human serum - the C9 depletion test (C9DT) -, was devised. C9DT showed significantly lower values for patients with systemic lupus erythematosus and rheumatoid arthritis than for control patients.

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AntigensArthritis, RheumatoidComplement ActivationComplement C9Complement Pathway, AlternativeDinitrophenolsHot TemperatureHumansImmunochemistryImmunoglobulin GInulinSerum Albumin, BovineZymosanJournal Article

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K Yoshida, Y Yukiyama, S Hirose. 1984. An immunochemical method for assessing the function of the alternative complement pathway.. https://doi.org/10.1159/000233609

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