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A cold stage for the Philips EM300 electron microscope.

K A Taylor·R A Milligan·C Raeburn·P N Unwin
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Abstract

A cold stage has been constructed for the Philips EM300 (and EM301) electron microscopes for investigating the structure of frozen-hydrated biological specimens. The stage entails minimal alterations to the instrument and is capable of a resolution better than 10 A at the normal operating temperature of -120 degrees C. Frozen specimens can be readily exchanged without condensation or warming up, and maintained in the stage over periods of several hours without detectable deterioration.

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FreezingMicroscopy, ElectronJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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K A Taylor, R A Milligan, C Raeburn, P N Unwin. 1984. A cold stage for the Philips EM300 electron microscope.. https://doi.org/10.1016/0304-3991(84)90197-9

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