Dynamical electron scattering from negatively stained protein microcrystals.

Abstract

Multislice n-beam dynamical calculations have been carried out at 20 and 15.6 A resolution to simulate the electron scattering from a negatively-stained protein microcrystal using a model based on the crystal structure of E. coli matrix porin. Images at t = 100 A, i.e. the thickness of reconstituted vesicle preparation, correspond to the projected potential of the kinematical structure. The kinematical model is valid at least to t = 500 A, beyond which the projected-image symmetry does not correspond to the actual structure. If a crystallographic residual limit R = 0.20 is imposed, the electron diffraction intensity data correspond to the kinematical data up to t = 700 and 1000 A, respectively, for 15.6 and 20 A resolution.