PubMed · 6534609
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H Clop, P Dublineau, G Larbi. [Implants and glaucoma].. https://pubmed.ncbi.nlm.nih.gov/6534609/
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PURPOSE: Traumatic cataract is a common blinding eye disease after ocular trauma, and its pathogenesis is closely related to lens epithelial cell dysfunction, while the definite molecular regulatory mechanism between upstream transcription factor and downstream target gene remains poorly clarified. This study aimed to clarify the role and molecular mechanism of the krüppel-like factor 5 (KLF5)/ thrombosponin 1 (THBS1) axis in regulating proliferation, migration, epithelial-mesenchymal transition and autophagy of lens epithelial cells in traumatic cataract, and to explore its potential clinical therapeutic value. METHODS: The GSE295383 dataset in the gene expression omnibus (GEO) database was downloaded, and the differentially expressed genes (DEGs) were screened by linear models for microarray data (limma) package of R language. Combined with Weighted gene co-expression network analysis (WGCNA), the gene co-expression network was constructed and the key modules were screened. Gene ontology (GO), kyoto encyclopedia of genes and genomes (KEGG) and gene set enrichment analysis (GSEA) combined with human transcription factor target (hTFtarget) and JASPAR databases were used to predict the upstream transcription factors of THBS1. Subsequently, SRA01/04 cells were induced with transforming growth factor-beta 2 (TGF-β2) to construct a cataract cell model. RESULTS: THBS1 and KLF5 were highly expressed in LECs exposed to TGF-β2. KLF5 could activate THBS1 transcription by binding to THBS1 promoter - 174 to -165 sites. Knockdown of THBS1 inhibited TGF-β2-induced viability, proliferation, migration, and altered the expression of epithelial-mesenchymal transition (EMT)- and autophagy-related markers in LECs. Knockdown of KLF5 downregulated THBS1 expression and produced a similar inhibitory effect, while overexpression of THBS1 reversed the effect of KLF5 knockdown. CONCLUSIONS: This study demonstrated that KLF5 promoted the proliferation, migration, and EMT‑associated molecular changes of LECs in traumatic cataract through transcriptional activation of THBS1, and regulated the expression of autophagy‑related markers in LECs, suggesting that KLF5/THBS1 axis might be a potential target for the treatment of traumatic cataract.
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We have previously reported the formation of valine hydroperoxides and aldehydes from hydroxyl-radical attack on free valine and protein molecules. We have also demonstrated that the major degradation products of valine hydroperoxides by several biochemical and cellular systems are the corresponding hydroxides, and therefore proposed that hydroxyvalines may serve as useful in vivo markers for studying protein oxidation. Here we have undertaken the structural elucidation of the oxidation products of leucine, another amino acid which is very susceptible to peroxidation. Hydroxyl-radical (HO.) attack on l-leucine in the presence of oxygen, followed by NaBH4 reduction, gave rise to five major oxidation products which have been isolated and identified. On the basis of chemical and spectroscopic evidence, the five products have been identified as (2S)-gamma-hydroxyleucine, (2S,4S)-delta-hydroxyleucine, (2S,4R)-delta-hydroxyleucine, (2S,4R)-4-methylproline (trans-4-methyl-l-proline) and (2S,4S)-4-methylproline (cis-4-methyl-l-proline). The three hydroxyleucines have been confirmed to be the reduction products of the corresponding hydroperoxyleucines, while the two proline analogues are from reduction of their corresponding cyclic Schiff bases. By HPLC analysis using post-column o-phthaldialdehyde derivatization, we have detected hydroxyleucines in the hydrolysates of tripeptides and proteins which had been gamma-radiolysed and treated with NaBH4. Furthermore, we demonstrate the occurrence of the hydroxyleucines on proteins in physiological and pathological samples. Hydroxyleucines, like hydroxyvalines, may provide useful in vivo markers for studying protein oxidation. In the present study we also investigated the competition between leucine, valine and phenylalanine for HO., and proposed a possible radical-transfer process in such free-radical reactions.