G2 cells: progression delay and survival.

M H Schneiderman; G S Schneiderman

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PubMed · 6539484

G2 cells: progression delay and survival.

M H Schneiderman·G S Schneiderman

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Abstract

The progression of Chinese hamster ovary (CHO) G2 cells into mitosis and their survival was measured after X-ray doses up to 4.0 Gy. S-phase cells were prevented from reaching mitosis by labeling with 125IUdR for 10 min prior to irradiation of the exponentially growing monolayer of cells. Mitotic cells, located past the radiation-induced division delay transition point, did not suffer a delay and were selected separately prior to the recovery of the G2 cells. The results show that (1) up to 400 min after radiation only 55% of the G2 cells recovered after about 2.5 Gy; (2) the progression delay of the G2 cells that recovered was 52.5 min/Gy; and (3) the survival curve D0 for these cells, 2.45 Gy, indicated a radioresistant population.

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Animals Cell Survival Cricetinae Cricetulus Dose-Response Relationship, Radiation Female Interphase Mitosis Ovary Time Factors Journal Article Research Support, U.S. Gov't, P.H.S.

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M H Schneiderman, G S Schneiderman. 1984. G2 cells: progression delay and survival.. https://pubmed.ncbi.nlm.nih.gov/6539484/

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