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Lipid bilayer dynamics and rhodopsin-lipid interactions: new approach using high-resolution solid-state 13C NMR.

M D Sefcik·J Schaefer·E O Stejskal·R A McKay·J F Ellena·S W Dodd·M F Brown
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Abstract

High-resolution, solid-state 13C NMR spectra have been obtained for unsonicated multilamellar dispersions of 1,2-dilauryl-sn-glycero-3-phosphocholine (DLPC), recombinant membranes containing DLPC and rhodopsin, and native retinal rod disk membranes. The roles of 1H dipolar decoupling, 1H-13C cross-polarization, and magic-angle sample spinning have been investigated. Rotating-frame 13C relaxation times have been measured and are discussed in terms of lipid bilayer dynamics and rhodopsin-lipid interactions.

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Fatty Acids, UnsaturatedLipid BilayersMagnetic Resonance SpectroscopyPhospholipidsRetinal PigmentsRhodopsinStructure-Activity RelationshipJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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M D Sefcik, J Schaefer, E O Stejskal, R A McKay, J F Ellena, S W Dodd, M F Brown. 1983-08-12. Lipid bilayer dynamics and rhodopsin-lipid interactions: new approach using high-resolution solid-state 13C NMR.. https://doi.org/10.1016/0006-291x(83)90668-x

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