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An inexpensive, microcomputer-based, video densitometer for quantitating thin-layer chromatographic spots.

T S Ford-Holevinski·B W Agranoff·N S Radin
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Abstract

An inexpensive video densitometer based on an Apple II microcomputer using commercially available components was developed. The device was used to quantitate charred lipid and colored spots on thin-layer chromatography plates. The method is useful for lipid samples weighing 0.1 to 4 micrograms on high-performance plates and 1 to 20 micrograms on ordinary plates. The software automatically corrects for baseline drift due to dirt or nonuniformity in the plate.

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CeramidesCholesterolChromatography, Thin LayerComputersDensitometryMicrocomputersJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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T S Ford-Holevinski, B W Agranoff, N S Radin. 1983-07-01. An inexpensive, microcomputer-based, video densitometer for quantitating thin-layer chromatographic spots.. https://doi.org/10.1016/0003-2697(83)90435-9

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