Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies.

G E Martorana; E Meucci; G A Miggiano; A Mordente; A Castelli

PubMed · 6654647

Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies.

G E Martorana·E Meucci·G A Miggiano·A Mordente·A Castelli

Abstract

Very low amounts of ascorbic acid modify alkaline phosphatase fluorescence, absorption and enzymatic activity. A strong quenching of enzyme, tryptophan and tyrosine emission together with evident alterations of the protein absorption characteristics are observed. The catalytic activity inhibition probably reflects a perturbation of the active site environment due to the interaction of ascorbic acid with enzyme aminoacyl residues.

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G E Martorana, E Meucci, G A Miggiano, A Mordente, A Castelli. Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies.. https://pubmed.ncbi.nlm.nih.gov/6654647/

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