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PubMed · 6685749

A rapid, isocratic method for phospholipid separation by high-performance liquid chromatography.

T L Kaduce·K C Norton·A A Spector
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Abstract

A rapid, isocratic method for separating the most prevalent phospholipids by high-performance liquid chromatography is described. Baseline resolution of phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, lysophosphatidylcholine, and sphingomyelin is achieved in less than 40 min on a silica column. Lipids are injected in 10 microliter of chloroform-diethyl ether 1:2 (v/v) and eluted with a solvent mixture of acetonitrile-methanol-sulfuric acid 100:3:0.05 (v/v/v) at a flow rate of 1 ml/min. Neutral lipids and cardiolipin elute with the solvent front. Chromatography of a radioactive cell lipid extract indicates a recovery of better than 97%. The procedure is sensitive enough to permit the analysis of the main phospholipids present in a monolayer culture containing about 100 micrograms of cell protein.

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AnimalsAortaCattleChromatography, High Pressure LiquidEndotheliumFatty AcidsMuscle, Smooth, VascularPhosphatidylcholinesPhospholipidsPlantsGlycine maxJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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T L Kaduce, K C Norton, A A Spector. 1983. A rapid, isocratic method for phospholipid separation by high-performance liquid chromatography.. https://pubmed.ncbi.nlm.nih.gov/6685749/

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