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A simplified method to determine binding parameters in a two-site case using linear regression.

V Guardabasso·F Benfenati
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Abstract

A program using a simplified method to compute estimates of binding parameters in a system with two independent classes of binding sites is presented. An iterative method is used to approximate step-by-step the two lines describing the binding activities separately, starting from a curvilinear Scatchard or Hofstee plot. This method is designed for use on microcomputers with graphic facilities and on programmable hand-held calculators.

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V Guardabasso, F Benfenati. 1983. A simplified method to determine binding parameters in a two-site case using linear regression.. https://doi.org/10.1016/0010-468x(83)90044-2

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