PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 6776159

Haemagglutination kinetics using a continuous-flow system.

P Rouger·P Gane·C Salmon
View original source ↗Export metadata ↓

Abstract

An automated technique for measuring haemagglutination kinetics is described. Equipment used in this test is very simple and already present in most blood transfusion centres. This method may be used with advantage to differentiate homozygous and heterozygous subjects in Rhesus, MNSs, and Kell systems, and to study antigen variations, especially in genetic investigations.

Explore related subjects

HemagglutinationHumansKell Blood-Group SystemKineticsMNSs Blood-Group SystemMethodsRh-Hr Blood-Group SystemJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

P Rouger, P Gane, C Salmon. 1980. Haemagglutination kinetics using a continuous-flow system.. https://doi.org/10.1136/jcp.33.9.885

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1991

Characteristics of hemolytic and hemagglutinating activities of Treponema denticola.

Treponema denticola, a suspected periodontal pathogen, was shown to agglutinate and lyse human red blood cells. Both activities were cell-associated, heat-labile, and produced during the exponential growth phase. Hemolytic activity was time-dependent and required incubation at 37 degrees C; further incubation at 4 degrees C increased the hemolysis. Hemagglutination was reduced in the presence of D-glucosamine, EDTA or sodium salicylate, whereas hemolytic activity was affected by calcium chloride, magnesium chloride, EDTA or sodium salicylate. These biological activities may favor a higher concentration of hemin-containing compounds in periodontal sites, and therefore represent additional potential virulence mechanisms.

Hemagglutination↗
PubMed1989

Extracellular and surface-bound biological activities of Vibrio fluvialis, Vibrio furnissii and related species.

Twenty-seven Vibrio strains were assessed for virulence-associated biological activities, including iron chelation, hydrolases, haemolysis and haemagglutination. All strains hydrolysed DNA, chitin, gelatin and casein, produced siderophores, and lysed red blood cells. All V. fluvialis, V. cholerae and V. mimicus strains exhibited diverse lipolytic activity distinct from more discriminate lipolysis by V. furnissii. V. furnissii manifested fibrin and mucin hydrolysis but no phosphate or esculin hydrolysis, for which V. fluvialis varied. No strains hydrolysed urea, alginate or keratin. V. fluvialis, V. furnissii and V. mimicus strains failed to exhibit the mannose-sensitive haemagglutination typical of V. cholerae. Some activities may distinguish otherwise phenotypically similar species. Species tested commonly possessed biological activities that may contribute to virulence, although there was no apparent correlation with isolation from human sources.

Hemagglutination↗
PubMed1989

Inhibition of phytoagglutinin potency of Korean vicia unijuga by human urine.

Human urines are often examined for individual identification in medicolegal field, therefore, authors attempted to classify the urines by inhibition of agglutination potency of phytoagglutinin Korean vicia unijuga which is known as a panphytoagglutinin to human red blood cells, and demonstrated that some individuals excrete urine which has ability to interfere with the agglutination potency of vicia extracts. The inhibiting substance appears to be either a protein or a glycoprotein, for it can be eliminated by boiling the urine sample. This substance is not present in human serum, semen and saliva. Presence or absence of the inhibiting substance in the urine has no relation to the individual's blood type and a sample urine spot test yielded a high rate of inhibitory property.

Hemagglutination↗