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PubMed · 6809079

Human blood monocytes and platelets share a cell surface component.

J J Burckhardt·W H Anderson·J F Kearney·M D Cooper
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Abstract

We describe a surface determinant shared by human monocytes and cells of the megakaryocytic axis that has been identified using a mouse monoclonal antibody. This monocyte-platelet antigen (MPA) is expressed on all (greater than 99%) of peripheral blood monocytes, platelets, and megakaryocytes. It is also expressed weakly on the monocytic cell line U937 and the promyelocytic line HL60 and is present on cells from 3 of 4 AML patients examined. It is absent from polymorphonuclear leukocytes, T and B lymphocytes, erythrocytes, and a panel of hematopoietic cell lines. MPA is stripped from monocyte membranes with pronase and is reexpressed overnight. The determinant is carried on a noncovalently linked biomolecular complex with molecular weights of 93,000 and 135,000.

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AnimalsAntibodies, MonoclonalAntigens, SurfaceBlood PlateletsHaplorhiniHumansLeukemia, MyeloidMiceMice, Inbred BALB CMolecular WeightMonocytesPronaseJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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J J Burckhardt, W H Anderson, J F Kearney, M D Cooper. 1982. Human blood monocytes and platelets share a cell surface component.. https://pubmed.ncbi.nlm.nih.gov/6809079/

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