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Cell type conversion in a mouse melanoma cell clone.

S Sato·T Takeuchi
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Abstract

A melanoma cell clone was isolated from cultured B16 mouse melanoma cells. This clone, conv, which was characterized by rounded and spindle-shaped cell morphology, was not highly melanotic under the usual culture condition but had high tyrosinase (dopa oxidase) activity. When the cells were seeded to form colonies on a plastic culture dish in Eagle's minimum essential medium supplemented with 10% bovine calf serum, two kinds of cell types always appeared. One was cytochemically dopa-positive and spindle-shaped (S type cell) with the same phenotypes as those of the parental cells. The other was dopa-negative and fibroblastlike (F type cell) containing no melanosomes. It was observed that the conversion from S type to F type occurred with a high frequency. The conversion from F type to S type also occurred but with a low frequency.

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AnimalsCell DifferentiationClone CellsMelaninsMelanomaMiceMonophenol MonooxygenaseNeoplasms, ExperimentalPhenotypeComparative StudyJournal Article

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S Sato, T Takeuchi. 1982. Cell type conversion in a mouse melanoma cell clone.. https://doi.org/10.1007/bf02796420

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