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A solid-phase ELISA for human galactosyltransferase.

B Verdon·T Mandel·E G Berger·H Fey
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Abstract

Monospecific rabbit anti-human milk galactosyltransferase antibodies were used to develop a solid-phase enzyme-linked immunosorbent assay (ELISA) for the human milk enzyme. The assay was based on competition with galactosyltransferase-phosphatase conjugate. The detection limit of the standard curve was 30 ng/ml. Galactosyltransferase was quantified in dialyzed milk samples by ELISA with variation coefficients of less than 7.7% and between 6.8 and 18.8% within and between assays, respectively. The average content was 61 +/- 19 microgram/ml. The assay proved suitable for quantitation of partially purified enzyme preparations in body fluids.

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AnimalsAntibodies, MonoclonalAntigensEnzyme-Linked Immunosorbent AssayGalactosyltransferasesHumansMilk, HumanRabbitsJournal ArticleResearch Support, Non-U.S. Gov't

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B Verdon, T Mandel, E G Berger, H Fey. 1982-11-26. A solid-phase ELISA for human galactosyltransferase.. https://doi.org/10.1016/0022-1759(82)90073-4

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