Temporal relationship between primary injection and cyclophosphamide administration necessary for the inhibition of the priming for a secondary response.

J Gras; T Tilló

doi:10.1016/0769-2625(81)90023-4

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Temporal relationship between primary injection and cyclophosphamide administration necessary for the inhibition of the priming for a secondary response.

J Gras·T Tilló

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Abstract

Using the mouse PFC anti-SRBC response, the temporal relationship between primary antigen dose and cyclophosphamide (CP) administration necessary for the inhibition of priming for the secondary IgG response was studied. The administration of CP 1 h after the primary or secondary antigen injection inhibits the responses in both cases. The administration of CP 1 h after the primary antigen injection does not inhibit priming for the secondary response; this priming is inhibited if CP is administered at 12 h or more, and the maximal degree of inhibition is induced when CP is administered 7 or 9 days after the primary antigen injection. Therefore, the proliferation stage of mature B cells to IgM secretion is not necessary for this priming. It is suggested that the switch from IgM to IgG takes place in a proliferative stage of B-cell precursors in presence of the antigen.

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Animals Antibody Formation B-Lymphocytes Cyclophosphamide Erythrocytes Immunoglobulin G Immunoglobulin M Mice Sheep Time Factors Journal Article Research Support, Non-U.S. Gov't

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J Gras, T Tilló. Temporal relationship between primary injection and cyclophosphamide administration necessary for the inhibition of the priming for a secondary response.. https://doi.org/10.1016/0769-2625(81)90023-4

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