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PubMed · 6990864

Quantitative, radial diffusion slide assay for staphylocoagulase.

J D Kohl·M G Johnson
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Abstract

A simple, quantitative radial diffusion assay for staphylocoagulase in culture fluids, using microscope slides coated with a thin layer of agar containing plasma and fibrinogen, was developed. No prior purification of the enzyme was needed, and only small quantities, 7 microliter, were required for each test. This method is particularly suitable for objectively comparing the relative amounts of coagulase produced by different cultures of Staphylococcus aureus.

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AnimalsBacteriological TechniquesBloodCoagulaseCulture MediaFemaleRabbitsStaphylococcus aureusSwineComparative StudyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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J D Kohl, M G Johnson. 1980. Quantitative, radial diffusion slide assay for staphylocoagulase.. https://doi.org/10.1128/aem.39.2.339-341.1980

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