PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 7008176

Solid phase ELISA for serum ferritin.

M Pagé·L Thériault·M Nilsson
View original source ↗Export metadata ↓

Abstract

We describe a solid-phase enzyme immunoassay for human serum ferritin, with the use of polyethylene glycol to shorten the reaction. The amount of bound enzyme-antibody conjugate used as a second site antibody is proportional to the ferritin titre in the assay. This method is fast, and the reagents are stable for many months at 4 degrees C. The lowest detectable concentration was 5 micrograms/l. Interassay coefficients of variation were 9.3 and 12.2%, respectively, at ferritin concentrations of 100 micrograms/l and 200 micrograms/l. The data also show that polyethylene glycol accelerates antigen-antibody complex formation in the solid phase. An assay may be completed in 5 h.

Explore related subjects

Enzyme-Linked Immunosorbent AssayFerritinsHumansImmunoenzyme TechniquesPolyethylene GlycolsTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

M Pagé, L Thériault, M Nilsson. 1980. Solid phase ELISA for serum ferritin.. https://doi.org/10.1080/00365518009091976

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2002

High serum concentrations of dimeric inhibins A and B in normal newborn girls.

OBJECTIVE: To establish the serum pattern of dimeric inhibins in normal girls during the newborn period and to examine its relationship with the postnatal gonadotropic surge. DESIGN: Retrospective study. SETTING; Division of endocrinology of a children's hospital. PATIENT(S): Thirty-one girls 4 to 65 days of age. MAIN OUTCOME MEASURE(S): Serum levels of FSH, LH, and inhibin A and B. RESULT(S): High serum concentrations of both dimeric inhibins were detected during the first weeks of life. Concentrations tended to decrease through the second month of age. Inhibin A and B concentrations positively correlated with age only during the first 2 weeks of life (r =.57, P<.05 and r =.70, P<.01, respectively). Inhibin A and B were positively and significantly correlated with LH (r =.57, P<.05 and r =.52, P<.05, respectively) and with each other (r =.68, P<.0001). No correlation was found between dimeric inhibins and FSH. CONCLUSION(S): The high inhibin concentrations observed in newborn girls, which indicate advanced follicular development and granulosa cell differentiation, may be useful markers for congenital gonadal disorders.

Enzyme-Linked Immunosorbent Assay↗
PubMed2002

Matrix effects in applying mono- and polyclonal ELISA systems to the analysis of weathered oils in contaminated soil.

Commercial mono- and polyclonal enzyme-linked immunosorbent assay (ELISA) systems were applied to the on-site analysis of weathered hydrocarbon-contaminated soils at a former integrated steelworks. Comparisons were made between concentrations of solvent extractable matter (SEM) determined gravimetrically by Soxhlet (dichloromethane) extraction and those estimated immunologically by ELISA determination over a concentration range of 2000-330,000 mg SEM/kg soil dry weight. Both ELISA systems tinder-reported for the more weathered soil samples. Results suggest this is due to matrix effects in the sample rather than any inherent bias in the ELISA systems and it is concluded that, for weathered hydrocarbons typical of steelworks and coke production sites, the use of ELISA requires careful consideration as a field technique. Consideration of the target analyte relative to the composition of the hydrocarbon waste encountered appears critical.

Enzyme-Linked Immunosorbent Assay↗
PubMed2002

Definition of a type of abnormal vaginal flora that is distinct from bacterial vaginosis: aerobic vaginitis.

OBJECTIVE: To define an entity of abnormal vaginal flora: aerobic vaginitis. DESIGN: Observational study. SETTING: University Hospital Gasthuisberg, Leuven, Belgium. SAMPLE: 631 women attending for routine prenatal care or attending vaginitis clinic. METHODS: Samples were taken for fresh wet mount microscopy of vaginal fluid, vaginal cultures and measurement of lactate, succinate and cytokine levels in vaginal fluid. Smears deficient in lactobacilli and positive for clue cells were considered to indicate a diagnosis of bacterial vaginosis. Aerobic vaginitis was diagnosed if smears were deficient in lactobacilli, positive for cocci or coarse bacilli, positive for parabasal epithelial cells, and/or positive for vaginal leucocytes (plus their granular aspect). RESULTS: Genital complaints include red inflammation, yellow discharge, vaginal dyspareunia. Group B streptococci, escherichia coli, staphylococcus aureus and trichomonas vaginalis are frequently cultured. Vaginal lactate concentration is severely depressed in women with aerobic vaginitis, as in bacterial vaginosis, but vaginal succinate is not produced. Also in contrast to bacterial vaginosis, aerobic vaginitis produces a host immune response that leads to high production of interleukin-6, interleukin-1-beta and leukaemia inhibitory factor in the vaginal fluid. CONCLUSION: Aerobic vaginitis is associated with aerobic micro-organisms, mainly group B streptococci and E. coli. Its characteristics are different from those of bacterial vaginosis and elicit an important host response. The most severe form of aerobic vaginitis equals desquamative inflammatory vaginitis. In theory, aerobic vaginitis may be a better candidate than bacterial vaginosis as the cause of pregnancy complications, such as ascending chorioamnionitis, preterm rupture of the membranes and preterm delivery.

Enzyme-Linked Immunosorbent Assay↗