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PubMed · 7055899

Two serum pancreatic isoamylase determinations compared.

T Rammeloo·P M van Haard·M H Beunis
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Abstract

Serum pancreatic isoamylase activity was measured by a method involving inhibition of salivary isoamylase and by a well-known agarose electrophoretic method, modified by us. We saw changes in electrophoretic patterns of pancreatic isoamylase fractions after storage of serum samples for three weeks at 4-8 degrees C, but with the inhibition method no alterations in activities were found. Within-assay and between-assay imprecisions of both methods were about the same. Serum pancreatic amylase activities as measured by the inhibition method exceeded by about 10% those obtained by the electrophoretic method. The inhibition method seems to be a reasonable candidate for routine application, whereas the electrophoretic method is still time-consuming and requires special skill to perform. Some suggestions are given for improving the calibration of the inhibition method recommended by the manufacturer.

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Blood PreservationCold TemperatureElectrophoresis, Agar GelGlycoside HydrolasesHumansIsoamylasePancreasPancreatic DiseasesReagent Kits, DiagnosticReference ValuesSalivaTime FactorsComparative StudyJournal Article

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T Rammeloo, P M van Haard, M H Beunis. 1982. Two serum pancreatic isoamylase determinations compared.. https://pubmed.ncbi.nlm.nih.gov/7055899/

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