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PubMed · 7120367

Automated cell counting in tissue sections: a new approach by 'multiple grey-level analysis'.

D Wynford-Thomas·N Garrahan·B Jasani·E D Williams
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Abstract

A system is described for the automated quantitation in tissue sections of the follicular and stromal cell populations of the thyroid gland, using a computer-linked TV image analyser. The paper illustrates how the problems of variable staining intensity and clustering of images (nuclei) may be overcome by a novel approach, in which multiple analyses of each field are made at increasing grey-level thresholds, and then 'synthesized' by computer to give a composite image. Discrimination between cell (nuclear) types was by a single minimum width criterion. The results were highly reproducible and correlated well with counts obtained by a comparable manual method.

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AnimalsAutoanalysisCell CountComputersHyperthyroidismRatsThyroid GlandThyrotropinComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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D Wynford-Thomas, N Garrahan, B Jasani, E D Williams. 1982. Automated cell counting in tissue sections: a new approach by 'multiple grey-level analysis'.. https://doi.org/10.1111/j.1365-2818.1982.tb00411.x

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