A selection system specific for the Thy mutator phenotype.

M Meuth; O Gonçalves; P Thom

doi:10.1007/bf01538705

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A selection system specific for the Thy mutator phenotype.

M Meuth·O Gonçalves·P Thom

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Abstract

Thy- mutants, in addition to being resistant to arabinosyl cytosine (arcC), show cross-resistance to 5-fluorouracil (5FU). When Chinese hamster ovary (CHO) cells were exposed to a selection system using both araC and 5FU, the resistant clones isolated were identical to thy- mutants by the following criteria: (1) all were auxotrophic for thymidine with a high reversion frequency to thymidine prototrophy; (2) those tested had a high level of dCTP relative to wild-type cells, while dTTP and dATP levels were unaffected, and (3) all tested had a 7- to 50-fold higher rate of spontaneous mutation than the wild-type strain for at least one independent genetic marker. Although spontaneous thy- mutants were rare, the frequencies of thy- mutants in untreated and mutagenized cultures are consistent with the conclusion that the thy- phenotype is the consequence of a single mutation in CHO cells.

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Animals Cell Line Cell Separation Cricetinae Cricetulus Cytarabine Deoxyribonucleotides Drug Resistance Female Fluorouracil Mutation Ovary Thymidine Journal Article Research Support, Non-U.S. Gov't

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M Meuth, O Gonçalves, P Thom. 1982. A selection system specific for the Thy mutator phenotype.. https://doi.org/10.1007/bf01538705

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