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PubMed · 722048

Device for quantitative cytochemistry: a computerized scanning microdensitometer 'grain counter'.

N S Markovic·L E Lipkin·O S Markovic·M J Wade
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Abstract

A computerized scanning microdensitometer and autoradiographic grain counter was able to provide quantitative data on the cytochemical final reaction product formed within a single cell and also quantitate the kinetics of its formation. Optical density and area measurements were performed on hundreds of leukocytes from slides previously stained to demonstrate any one of a variety of reactions. These included cellular glycogen, lipids, peroxidase, esterases, alkaline phosphatase, and acid phosphatase. In addition to these slide studies, chamber studies with an adapted Dvorak-Stotler Chamber allowed the measurement of enzyme kinetics within single cells.

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AutoradiographyComputersEsterasesGlycogenHistocytochemistryHumansLeukocytesLipidsPeroxidasesJournal Article

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N S Markovic, L E Lipkin, O S Markovic, M J Wade. 1978. Device for quantitative cytochemistry: a computerized scanning microdensitometer 'grain counter'.. https://doi.org/10.1177/26.10.722048

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