PubMed · 7253281
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H Inoue. 1980. [Hitachi 716 automatic analyzer (author's transl)].. https://pubmed.ncbi.nlm.nih.gov/7253281/
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An analytical method is presented to determine glycerol concentrations and stable isotope tracer enrichments in human plasma after intravenous tracer infusion in a single analytical run, using gas chromatography coupled to mass spectrometry. The method uses an internal standard, which is also a stable isotope labeled form of glycerol. Three substances were tested as model compounds viz. [2-13C]glycerol, and [1,2,3-13C3]glycerol, and [1,1,2,3, 3-2H5]glycerol. Any combination of two can be used (one as internal standard, one as tracer), even if overlapping of the mass spectra occurs. The method is precise (recovery of spiked glycerol and tracer are, respectively, 99.7 and 99.8%) and reproducible (intraassay variation <1.5%, interassay variation <6%) and needs only a small amount of plasma (100 microl).
The increasing use of the potent hallucinogenic drug D-lysergic acid diethylamide (LSD) makes the application of highly sensitive analytical techniques necessary. For an effective analysis of biological specimens a limit of detection in the sub-ng/ml range is required. Using capillary electrophoresis (CE), with laser-induced fluorescence detection it was possible to determine 0.1-0.2 ng LSD/ml blood. The conventionally used immunoassays and gas and high-performance liquid chromatographic methods have cut-off values and limit of detection, respectively, in the same range. Only tandem mass spectrometry techniques can be more sensitive. Therefore, CE can be considered a good complementary method to conventional immunological and chromatographic techniques for the forensic analysis of biofluids.