PubMed HealthSearch

PubMed · 727451

Polyacrylamide gel electrophoresis with optical scanning, using multiphasic buffer systems: the stack.

Abstract

The source did not provide an abstract. Follow the original record for more information.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

B Chen, D Rodbard, A Chrambach. 1978. Polyacrylamide gel electrophoresis with optical scanning, using multiphasic buffer systems: the stack.. https://doi.org/10.1016/0003-2697(78)90388-3

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Lipases of Fonsecaea pedrosoi and Phialophora verrucosa.

Lipase activity was demonstrable titrimetrically in the culture filtrates of Fonsecaea pedrosoi and Phialophora verrucosa on the 6th day of incubation reaching a peak on the 15th and 12th days respectively for the two fungi. Purified lipases of F. pedrosoi and P. verrucosa, with specific activities of 36.0 and 39.4 fold increase respectively, were obtained by cold acetone extraction, gel filtration followed by ion exchange chromatography. The lipases had the same optimum pH (5.5) and temperature (35 degrees C). The molecular weights of the lipases of F. pedrosoi and P. verrucosa, estimated by gel filtration on Sephadex G-100, were 25,000 and 20,000, respectively and the enzymes showed broad substrate specificity. The possible role of lipase in the pathogenesis of infection caused by the fungi is discussed.

Electrophoresis, Disc

[Comparison of automated micro-disc electrophoresis for the analysis of urine proteins with a conventional system].

Recently a new system for analyzing urinary proteins due to their molecular weight distribution has been introduced. The whole system is automatically driven and uses a silver staining. Due to the small amount of protein necessary for analysis native urine samples can be used. The data presented here show that the automatic micro-disc-electrophoresis (Phast-system) is very useful for any routine testing due to the quick (2 hours) and easily obtainable results with a very high reproducibility. The clinical relevance of urinary protein analysis seems to be much higher using this system.

Electrophoresis, Disc