A rapid experimental method to study primary embryonic induction.

L Gualandris; A M Duprat

doi:10.1111/j.1432-0436.1981.tb01183.x

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A rapid experimental method to study primary embryonic induction.

L Gualandris·A M Duprat

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Abstract

The technique described here is a combination of the sandwich-method and cell culture. It allows one to know quickly whether induction occurred or not (48 h if cell spreading is considered, 4-5 days if morphological differentiation is observed). The dissociation of the explants soon after induction does not disturb their inductive pattern. Moreover, this method allows a daily observation of the morphological events and study at the cellular and/or molecular level.

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Animals Cell Aggregation Cell Differentiation Culture Techniques Female Gastrula Neural Crest Pleurodeles Journal Article Research Support, Non-U.S. Gov't

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L Gualandris, A M Duprat. 1981. A rapid experimental method to study primary embryonic induction.. https://doi.org/10.1111/j.1432-0436.1981.tb01183.x

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