PubMed · 73888
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
E N Wardle. 1976-08-21. Immunosuppression by fatty acids.. https://doi.org/10.1016/s0140-6736(76)92446-6
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.
Three approaches for the synthesis of octadecadienoic acids with conjugated double bond systems are presented: synthesis of (10Z, 12Z)-octadecadienoic acid via an enyne-substructure; the use of an educt with a conjugated double bond system for the synthesis of (10E, 12E)-octadecadienoic acid; and the Suzuki cross coupling for the synthesis of (7E,9Z)-octadecadienoic acid.
Well-studied bacteria such as Bacillus subtilis and Escherichia coli each have only a single pathway for synthesis of the unsaturated fatty acids required to make functional membrane lipids. In marked contrast, unsaturated fatty acid synthesis in Pseudomonas aeruginosa proceeds by three distinct pathways.