PubMed · 7469130
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R Bookman. 1981. Dog dander extracts.. https://pubmed.ncbi.nlm.nih.gov/7469130/
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Restrictocin is a toxin produced by the fungus Aspergillus restritus. The DNA coding for restrictocin was isolated from the host by polymerase chain reaction and cloned into a T7 promoter-based expression vector. The protein was overproduced in Escherichia coli and remained insoluble in the cell in the form of inclusion bodies. Recombinant restrictocin was purified in large amounts, by a simple denaturation-renaturation protocol involving a redox system, with typical yields of 45 mg/l of original culture. Restrictocin could be secreted into the bacterial medium using ompA, pelB and LTB signal sequences. Among the three signal sequences, ompA was found to be the most efficient in secreting the recombinant protein. The protein secreted into the extracellular medium was properly processed as evident by the amino-terminal sequencing. Recombinant restrictocin was readily purified to homogeneity from either the medium or inclusion bodies by simple chromatographic techniques and was found to be functionally as active as the native fungal protein in inhibiting the eukaryotic translation.
Latex, a natural plant material, has gained increasing interest over the last years because of its allergenic potency inducing problems in medical professions as well as in daily life. Since 1979 reports on allergic spontaneous reactions induced by latex products (such as gloves, condoms, balloons, cofferdam) have increased steadily. Symptoms manifest clinically as contact urticaria, rhinoconjunctivitis, asthma or anaphylactic shock. Some of these patients may additionally have an alimentary allergy (avocado, banana, maron i.e.), possibly containing agents cross-reacting with latex. Pathophysiologically the allergy is IgE-mediated, because antigen-specific IgE antibodies have been found in vitro as well as in vivo. Health professionals, workers in gum manufacturing and individuals with spina bifida have been found to be at special risk for a latex allergy therefore, it is important that these persons at risk are recognized and occasionally tested prior to interventions or operations. Strict avoidance of the use of latex products is the primary preventive measure, which may occasionally be difficult, particularly in patients with respiratory diseases.
An antisense gene strategy was applied to suppress the 14-16 kDa allergen gene expression in maturing rice seeds. Gene constructs producing antisense RNAs of the 16 kDa allergen under the control of some rice seed-specific promoters were introduced into rice by electroporation. Immunoblot and RNA blot analyses of the seeds from the transgenic rice plants using the allergen-specific monoclonal antibody and a sequence-specific antisense RNA probe demonstrated that the 14-16 kDa allergen proteins and their transcripts of the seeds from several transgenic lines were present in much lower in amounts than those of the seeds from parental wild-type rice. The high levels of reduction observed were stably inherited in at least three generations.