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PubMed · 7509755

Correlation between esterase electrophoretic polymorphism and virulence-associated traits in extra-intestinal invasive strains of Escherichia coli.

P Goullet·B Picard·M Contrepois·J De Rycke·J Barnouin
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Abstract

The electrophoretic variations of carboxylesterase B and of esterases A, C and I, the presence of mannose resistant haemagglutinin, alpha-haemolysin, cytotoxic necrotizing factor type 1 (CNF1) and certain O antigens were compared in 150 strains of Escherichia coli responsible for extra-intestinal infections. Electrophoretic mobilities of outer membrane proteins (OMP) were also studied for strains belonging to O4, O6, O7, O8 and O75 serogroups. Fast migrating allozymes of carboxylesterase B (pattern B1) were correlated with slow migrating allozymes of esterase C, serogroups O7 and O8, lack of virulence factor, and particular OMP patterns, whereas slow migrating allozymes of carboxylesterase B (pattern B2) were correlated with fast migrating allozymes of esterase C, serogroups O2, O4, O6, O18 and O75, virulence factor production, and distinct OMP patterns. Allozymes of esterases A and I were not clearly correlated with the distribution of virulence factors. The pattern B2 was more strongly associated with CNF1 than with alpha-haemolysin and mannose resistant haemagglutinin. These results substantiate the view that the electrophoretic pattern B2 of carboxylesterase B identified most of the highly pathogenic strains implicated in extra-intestinal infection of humans.

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BacteremiaBacterial Outer Membrane ProteinsBacterial ProteinsBacterial ToxinsCarboxylesteraseCarboxylic Ester HydrolasesCytotoxinsElectrophoresis, Polyacrylamide GelEscherichia coliEscherichia coli InfectionsEscherichia coli ProteinsEsterasesHemagglutininsHemolysin ProteinsHumansIsoenzymesO AntigensPolymorphism, GeneticPolysaccharides, BacterialSerotypingUrinary Tract InfectionsVirulenceComparative StudyJournal Article

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P Goullet, B Picard, M Contrepois, J De Rycke, J Barnouin. 1994. Correlation between esterase electrophoretic polymorphism and virulence-associated traits in extra-intestinal invasive strains of Escherichia coli.. https://doi.org/10.1017/s0950268800057411

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