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PubMed · 7517958

Evaluation of a safe sputum processing method for detecting tuberculosis.

A Rattan·K Kishore·S Singh·M Jaber·I Xess·R Kumar
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Abstract

AIMS: To evaluate a safe sputum processing method for detection of tuberculosis in developing countries. METHODS: A sample processing method was developed in which acid fast bacilli were killed with 1% sodium hypochlorite and concentrated by flotation on a layer of xylene before staining by the Ziehl Neelsen or auramine O methods. RESULTS: Best results were obtained by auramine O staining after flotation. Staining by the Ziehl Neelsen method after flotation gave better results than direct Ziehl Neelsen staining without flotation. CONCLUSIONS: The flotation method with Ziehl Neelsen staining offers advantages for smear preparation in the tuberculosis control programmes of developing countries.

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Bacteriological TechniquesBenzophenoneidumHumansMycobacterium tuberculosisRandom AllocationSodium HypochloriteSpecimen HandlingSputumStaining and LabelingTuberculosis, PulmonaryXylenesJournal Article

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A Rattan, K Kishore, S Singh, M Jaber, I Xess, R Kumar. 1994. Evaluation of a safe sputum processing method for detecting tuberculosis.. https://doi.org/10.1136/jcp.47.5.411

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