Interstitial volume changes during spreading depression (SD) and SD-like hypoxic depolarization in hippocampal tissue slices.

J Jing; P G Aitken; G G Somjen

doi:10.1152/jn.1994.71.6.2548

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Interstitial volume changes during spreading depression (SD) and SD-like hypoxic depolarization in hippocampal tissue slices.

J Jing·P G Aitken·G G Somjen

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Abstract

1. Relative interstitial volume (ISV) was estimated from the concentration changes of iontophoretically administered tetramethyl- and tetraethylammonium (TMA+ and TEA+). Spreading depression (SD) was provoked by high K+, and hypoxic SD-like depolarization (HSD) was induced by withdrawing oxygen. 2. Probe ion concentrations increased dramatically and about equally during SD and HSD, except that in a few hypoxic trials signals became transiently smaller than control. Interstitial volume appeared to decrease on the average by approximately 70%. 3. The ISV that remains patent in CA1 region at the height of SD is < 4% of total tissue volume. Probe ions may occasionally have passed through cell membranes for a short time during hypoxic SD.

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Animals Cell Hypoxia Cortical Spreading Depression Culture Techniques Extracellular Space Hippocampus Ion Channels Membrane Potentials Potassium Rats Synaptic Transmission Journal Article Research Support, U.S. Gov't, P.H.S.

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J Jing, P G Aitken, G G Somjen. 1994. Interstitial volume changes during spreading depression (SD) and SD-like hypoxic depolarization in hippocampal tissue slices.. https://doi.org/10.1152/jn.1994.71.6.2548

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