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Quantitative micro P30 and reverse transcriptase assays for Moloney murine leukemia virus.

C A Wilson·M V Eiden·J W Marsh
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Abstract

An anti-P30 immunohistochemical and a reverse transcriptase assay for Moloney murine leukemia virus (MoMLV) are adapted to 96-well plates. The assay results are shown to be directly proportional to the number of infectious particles, and can therefore be used to estimate the infective titers of a virus preparation. The micro P30 assay yields a direct estimate of infectious centers, and the reverse transcriptase assay quantitates progeny from a single cycle of replication. The semi-automated nature of these assays is well suited to the analysis of a large number of samples and therefore permits the examination of the efficiency of the process of retroviral/MoMLV infection under varied times or conditions.

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3T3 CellsAnimalsCryopreservationMiceMoloney murine leukemia virusRNA-Directed DNA PolymeraseReproducibility of ResultsRetroviridae Proteins, OncogenicSafetySensitivity and SpecificityViral Envelope ProteinsViral Plaque AssayJournal Article

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C A Wilson, M V Eiden, J W Marsh. 1994. Quantitative micro P30 and reverse transcriptase assays for Moloney murine leukemia virus.. https://doi.org/10.1016/0166-0934(94)90093-0

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