Parameter sensitivity analysis for designing experiments in kinetics.

Abstract

For the Michaelis-Menten binding kinetics and for a two- and a three-compartmental model a parameter sensitivity analysis is performed to design experiments. According to the mathematical results in the case of the binding kinetics the substrate concentrations s approximately Km are optimal in view to the variance of the Michaelis constant Km. In the case of the two-compartment model the results of the calculations are tested by the use of computer-simulated data. The correspondence between the sensitivity and simulation results is satisfactory.