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PubMed · 754485

Immune-complex phagocytosis by human polymorphonuclear granulocytes.

L Molnár·J M Baló-Banga·A Horváth·J Leibinger·E Ablonczy·M Nováki
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Abstract

A new two-step centrifugation technique is described for the rapid preparation of viable pure polymorphonuclear granulocytes from 9.5 ml of venous blood. The phagocytosis of these cells was stimulated by soluble DNA-anti-DNA complexes, produced in vitro by incubating high mol. wt. DNA with sera obtained from patients with severe SLE. The measure of phagocytosis was the amount of nitroblue-tetrazolium (NBT), reduced by the active microphages. The reduced NBT was estimated by counting the formazane positive cells or by extracting and measuring it spectrophotometrically. The immune-complex phagocytosis of control human granulocytes showed a linear relationship with the cell count per reaction mixture and displayed a bell-shaped dose-response curve as function of the immune-complex. The immune-complex phagocytosis by SLE granulocytes was decreased by about 40 per cent as compared to normal granulocytes.

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Antigen-Antibody ComplexDNAHumansLupus Erythematosus, SystemicMethodsNeutrophilsNitroblue TetrazoliumPhagocytosisJournal Article

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L Molnár, J M Baló-Banga, A Horváth, J Leibinger, E Ablonczy, M Nováki. 1978. Immune-complex phagocytosis by human polymorphonuclear granulocytes.. https://pubmed.ncbi.nlm.nih.gov/754485/

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