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Protein A in Staphylococcus aureus isolates from pigs.

S Takeuchi·K Matuda·K Sasano
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Abstract

The presence and quantity of protein A in Staphylococcus aureus 147 isolates from the tonsils of healthy pigs were examined by three methods. Cell-bound protein A was detected in 71 (48%), 104 (71%) and 123 (84%) of 147 isolates by the slide hemagglutination test, microplate hemagglutination test and enzyme-linked immunosorbent assay (ELISA), respectively. Extracellular protein A was not detected in any isolates by the microplate hemagglutination test. When the quantity of cell-bound protein A in the isolates was determined by the ELISA, most of the isolates contained about 0.8 to 2.2 micrograms of protein A/ml in bacterial cell suspensions of a concentration of MacFarland No. 3.

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AnimalsEnzyme-Linked Immunosorbent AssayErythrocytesHemagglutination TestsSheepStaphylococcal Protein AStaphylococcus aureusSwineComparative StudyJournal Article

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S Takeuchi, K Matuda, K Sasano. 1995. Protein A in Staphylococcus aureus isolates from pigs.. https://doi.org/10.1292/jvms.57.581

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