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Basic blue 148: a rapid stain for T helper cells.

K Kass
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Abstract

After brief exposure to an aqueous solution of the oxazine textile dye C.I. basic blue 148 following fixation in 37% formalin, 95% ethanol and glacial acetic acid, T helper cell nuclei and cytoplasm in specimens of peripheral blood displayed a deep red-violet color. No other cell in normal blood or bone marrow specimens showed intense staining of this type. The total staining time is 1 min. Basic blue 148 stain is a promising technique for hematology and immunology laboratories as a rapid screening test for T helper cells in blood specimens using a microscopic slide and ordinary incandescent illumination.

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K Kass. 1995. Basic blue 148: a rapid stain for T helper cells.. https://doi.org/10.3109/10520299509108325

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