A signal sequence detection system using secreted protease activity as an indicator.

M Yokoyama-Kobayashi; S Sugano; T Kato; S Kato

doi:10.1016/0378-1119(95)00322-w

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A signal sequence detection system using secreted protease activity as an indicator.

M Yokoyama-Kobayashi·S Sugano·T Kato·S Kato

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Abstract

We have developed a novel expression vector, pSSD1, to detect a cDNA fragment encoding a secretory signal sequence (Ss). This vector carries a cDNA fragment encoding the protease domain of human urokinase-type plasminogen activator (u-PA) as a reporter gene under the SV40 early promoter. We inserted cDNA fragments encoding various Ss between the promoter and the reporter gene of pSSD1. These plasmids were introduced into monkey COS7 cells to produce chimeric proteins. Only when the Ss was intact, was fibrinolytic activity detected in the culture medium of the transfected cells. Thus, this system may be useful for detecting the cDNAs encoding secreted proteins or type-I membrane proteins.

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Amino Acid Sequence Animals Base Sequence Cell Line DNA, Complementary Fibrinolysis Gene Transfer Techniques Genetic Vectors Haplorhini Humans Molecular Sequence Data Recombinant Fusion Proteins Sequence Analysis Urokinase-Type Plasminogen Activator Journal Article

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M Yokoyama-Kobayashi, S Sugano, T Kato, S Kato. 1995-10-03. A signal sequence detection system using secreted protease activity as an indicator.. https://doi.org/10.1016/0378-1119(95)00322-w

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