PubMed · 76281
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
S S Ali. 1978. A modified method to avoid postcontamination.. https://pubmed.ncbi.nlm.nih.gov/76281/
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
A tumour sensitivity assay, the differential staining cytotoxicity (DiSC) assay, which has been shown to have potential in predicting tumour response to cytotoxic drugs, has been developed to investigate the radiosensitivity of peripheral blood lymphocytes isolated from patients with chronic lymphocytic leukaemia (CLL). The method involved irradiating isolated lymphocytes (0.64-20.48 Gy at 8 x 10(5) cells/ml) and incubating for 4 days. Subsequent radiation-induced cell kill was assessed by differential staining of dead and live cells and calculation of tumour cell viability. Factors affecting assay success rate and in vitro radioresponse were investigated. Results were shown to be reproducible both when repeat assays were performed on the same specimen on the same day, and on different specimens from the same patient after intervening periods of time. Greater than 1 day transit time (whole blood) or storage (separated cells in medium) was found to be detrimental to assay success, but pH of the medium (either pH 7.3 or 8) had little effect. An increased incubation period (from 4 to 7 days) slightly reduced cell survival, but the underlying radioresponsiveness of the cells did not change.
Introduction of computers and image analysis systems are gaining faster momentum in order to quantitate the assessment of cells for diagnosis and prognosis, and this system aims to relieve the operator from the tedium of microscopic observation and reduce operator bias and human error. This paper discusses the design and configuration of an interactive image analysis system built in the laboratory for the purpose of cell analysis and classification. The software developed to compute various textural and morphological parameters of cells on smear are briefly described. The results of the experiments carried out to classify normal and abnormal cells on cervical smear show 94 per cent success rate.
The diagnostic accuracy of the toluidine blue stain approximates that of the Papanicolaou stain in non-gynecologic cytology. In this study a sensitivity of 95.3 and 96.9 per cent was achieved by used of the toluidine blue and Papanicolaou stains respectively. The specificity for both stains was identical at 96.3 per cent. Given the high degree of accuracy achieved with the toluidine blue stain in this study, and given its numerous advantages over the Papanicolaou stain, it is suggested that consideration be given to using the toluidine blue stain routinely in non-gynecologic cytology to differentiate malignancy from benignancy.