Improved method for polyamine determination in TMV, a rod-shaped virus.

P Torrigiani; A L Rabiti; L Betti; F Marani; M Brizzi; N Bagni; A Canova

doi:10.1016/0166-0934(95)00008-i

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Improved method for polyamine determination in TMV, a rod-shaped virus.

P Torrigiani·A L Rabiti·L Betti·F Marani·M Brizzi·N Bagni·A Canova

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Abstract

Polyamines were measured in viruses using different techniques. An improved method of polyamine analysis is reported for tobacco mosaic virus (TMV), a rod-shaped virus (95% protein and 5% RNA), based on HPLC of sonicated PCA-treated highly purified virus suspensions. This method allowed higher and more reliable recovery of TMV polyamines (putrescine, spermidine and spermine) when compared to the HPLC of non-sonicated samples and to thin layer chromatography. It is suggested that sonication acts on PCA-precipitated protein aggregates causing the release of trapped polyamine molecules.

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Chromatography, High Pressure Liquid Perchlorates Polyamines Putrescine Spermidine Spermine Tobacco Mosaic Virus Journal Article Research Support, Non-U.S. Gov't

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P Torrigiani, A L Rabiti, L Betti, F Marani, M Brizzi, N Bagni, A Canova. 1995. Improved method for polyamine determination in TMV, a rod-shaped virus.. https://doi.org/10.1016/0166-0934(95)00008-i

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