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PubMed · 7643478

Transforming growth factor-beta 1 induces morphological changes accompanied by extracellular matrix reconstitution in cultures of avian retinal pigmented epithelial cells.

H Ando·R Kodama·Y Tomoda·G Eguchi
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Abstract

The mechanisms of cell differentiation have been extensively studied with an avian retinal pigmented epithelial cell (PEC) culture system. Transforming growth factor-beta (TGF-beta) has been reported to have stimulatory or inhibitory effects on differentiation depending on tissue type. Although the mechanism by which TGF-beta exerts diverse effects is unknown, changes in the extracellular matrix (ECM) are believed to be related to the effects of TGF-beta. Therefore, the effects of TGF-beta 1 on the cellular morphology and the distribution of ECM components such as laminin, tenascin, fibronectin, and types I and IV collagens were investigated in confluent cultures of differentiated PECs during a period of 6 days. We found that differentiated PECs with polygonal morphology and pigmented granules changed to pleomorphic and less pigmented cells 4 days after the addition of TGF-beta 1. These changes were preceded by changes in the distribution of ECM components, particularly laminin and tenascin after 2 days. The effects of TGF-beta on the differentiation of PECs are discussed.

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AnimalsCell DifferentiationCell SizeCells, CulturedChick EmbryoCulture MediaExtracellular MatrixExtracellular Matrix ProteinsFluorescent Antibody TechniquePigment Epithelium of EyeTransforming Growth Factor betaJournal ArticleResearch Support, Non-U.S. Gov't

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H Ando, R Kodama, Y Tomoda, G Eguchi. 1995. Transforming growth factor-beta 1 induces morphological changes accompanied by extracellular matrix reconstitution in cultures of avian retinal pigmented epithelial cells.. https://pubmed.ncbi.nlm.nih.gov/7643478/

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