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Characterization of phe B gene encoding catechol 2,3-dioxygenase.

T Nishihara·T Yamada·K Takano·S Osada·J Nishikawa·M Imagawa
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Abstract

DNA sequence of the phe B gene isolated from a chromosome of the phenol degrading bacterium Pseudomonas putida BH is identical to that of the dmp B gene from the phenol degradative plasmid, pVI150. Catechol 2,3-dioxygenase encoded by phe B showed similar substrate specificity to that of xyl E. However, Phe B has much smaller Km values than Xyl E, indicating that Phe B is useful for treatment of low concentrations of catechol derivatives in waste water.

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Biodegradation, EnvironmentalCatechol 2,3-DioxygenaseCatecholsDioxygenasesGenes, BacterialOxygenasesPlasmidsPseudomonas putidaSequence Analysis, DNASubstrate SpecificityComparative StudyJournal Article

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T Nishihara, T Yamada, K Takano, S Osada, J Nishikawa, M Imagawa. 1994. Characterization of phe B gene encoding catechol 2,3-dioxygenase.. https://doi.org/10.1111/j.1472-765x.1994.tb00938.x

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