Fluorescence-based signal amplification technology.

V L Singer; V B Paragas; K D Larison; K S Wells; C J Fox; R P Haugland

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PubMed · 7765427

Fluorescence-based signal amplification technology.

V L Singer·V B Paragas·K D Larison·K S Wells·C J Fox·R P Haugland

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Abstract

The ELF alkaline phosphate substrate can be used to fluorescently label a wide variety of biological targets. This substrate yields a bright, photostable yellow-green fluorescent precipitate at the site of enzymatic activity. ELF labeling can be as much as 40 times as bright and hundreds of times as photostable as labeling with conventional fluorophores and yields signals capable of very fine submicroscopic resolution. Signal development is also extremely rapid, making the signal amplification technology well suited for applications such as RNA in situ hybridization.

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Alkaline Phosphatase Animals Concanavalin A ErbB Receptors Fibroblasts Flow Cytometry Fluorescent Dyes Humans Immunoblotting Immunohistochemistry In Situ Hybridization Mice RNA, Messenger Spectrometry, Fluorescence Tumor Cells, Cultured Zebrafish Comparative Study Journal Article Review

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V L Singer, V B Paragas, K D Larison, K S Wells, C J Fox, R P Haugland. 1994. Fluorescence-based signal amplification technology.. https://pubmed.ncbi.nlm.nih.gov/7765427/

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