Low temperature cultivation--a step towards process optimisation.

R Weidemann; A Ludwig; G Kretzmer

doi:10.1007/bf00762385

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Low temperature cultivation--a step towards process optimisation.

R Weidemann·A Ludwig·G Kretzmer

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Abstract

Adherent recombinant BHK cells were cultivated at temperatures between 30 and 37 degrees C. Batch and repeated-batch-cultivations in a 2-litre bioreactor showed a significant influence on metabolism and cell growth. The low-temperature-cultivations showed a lower growth rate and a lower glucose consumption rate and, therefore, less lactate production. On the other hand, the maximum cell density and productivity seemed not to be affected by the temperature reduction.

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Animals Cell Division Cell Line Cold Temperature Cricetinae Culture Techniques Glucose Kidney Kinetics Lactates Temperature Time Factors Journal Article Research Support, Non-U.S. Gov't

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R Weidemann, A Ludwig, G Kretzmer. 1994. Low temperature cultivation--a step towards process optimisation.. https://doi.org/10.1007/bf00762385

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