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PubMed · 7853275

The hypoosmotic swelling test.

K Takahashi·A Abu-Musa·A Uchida·M Kitao
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Abstract

In the present study, the effect of the concentration of the hypoosmotic solution, incubation time and duration from sperm collection to incubation on the hypoosmotic swelling (HOS) test were evaluated. Sperm were obtained from 20 fertile donors. The most stable sperm tail swelling was obtained at 150 mOsm. Incubation for 30 minutes was sufficient for the evaluation of overall swelling but not for the evaluation of b- and g-type sperm swelling. This suggests that incubation for one hour is necessary to obtain stable results. Incubation for more than one hour did not affect the percentage or type of sperm swelling. The time from sperm collection to incubation did not affect the HOS test. The HOS test is practical and not affected by the duration of incubation or time from sperm collection to incubation.

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Evaluation Studies as TopicHumansHypotonic SolutionsIncubatorsMaleSemen PreservationSperm TailTime FactorsComparative StudyJournal Article

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K Takahashi, A Abu-Musa, A Uchida, M Kitao. 1994. The hypoosmotic swelling test.. https://pubmed.ncbi.nlm.nih.gov/7853275/

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