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Spatial and temporal changes in signal transduction pathways during LTP.

K L Thomas·S Laroche·M L Errington·T V Bliss·S P Hunt
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Abstract

Following LTP induction in freely moving rats, in situ hybridization revealed discrete changes in the expression of one isoform in each of four families of serine/threonine kinases constitutively expressed in the dentate gyrus of the hippocampus. Expression of the alpha isoform of CaMKII showed a transient increase over the soma and a more persistent increase over the dendritic field of dentate granule cells. Of the PKC isoforms, only gamma PKC was up-regulated substantially 2 hr after LTP induction, declining to control levels 48 hr later. An increase in the expression of mRNA for ERK2 and raf-B was seen at 24 hr only. These results show that, during the maintenance phase of LTP in the hippocampus, there are selective increases in the expression of serine/threonine kinases and that these increases have specific and characteristic temporal and spatial profiles.

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AnimalsCalcium-Calmodulin-Dependent Protein KinasesDendritesGene Expression RegulationGenes, Immediate-EarlyGranulocytesHippocampusIsoenzymesLong-Term PotentiationProtein Kinase CRNA, MessengerRatsRats, Sprague-DawleySignal TransductionTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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K L Thomas, S Laroche, M L Errington, T V Bliss, S P Hunt. 1994. Spatial and temporal changes in signal transduction pathways during LTP.. https://doi.org/10.1016/0896-6273(94)90040-x

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